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TECHSPEC® components are designed, specified, or manufactured by Edmund Optics. TECHSPEC® 组件由爱特蒙特光学设计、规定或生产。 了解更多

475nm中心波长,50nm带宽,最小光密度6,荧光滤光片

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产品编码 #86-342 6-10 工作日
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RMB 2,194.69
数量 1-5
RMB 2,194.69
数量 6+
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Product Details

类型:
Bandpass Filter

Physical & Mechanical Properties

直径 (mm):
12.50 +0.00/-0.10
有效孔径 CA(mm):
8.69
厚度容差 (mm):
±0.1
构造 :
Mounted in Black Anodized Ring
物理的承受能力:
Adhesion per MIL-PRF-13830B, Section C.4.5.12
Moderate abrasion per MIL-PRF-13830B, Section C.4.5.11
Cleaning per MIL-C-48497A Section 4.5.4.2
基片厚度(mm):
2.00 ±0.25

Optical Properties

入射角 (°):
0 ±5
带宽 (nm):
50.00
OD 6 阻断波长范围 (nm):
515.5 - 565.5
光密度 OD(平均):
≥6.0
平均透射率:
>93 over Bandwidth
中心波长 CWL (nm):
475.00
半峰全宽 FWHM (nm):
56.00
基底: Many glass manufacturers offer the same material characteristics under different trade names. Learn More
Fused Silica (Corning 7980)
涂层:
Hard Coated
表面质量:
60-40
透射率 (%) :
>93
阻断波长范围 (nm):
250 - 1100
传输波前,RMS:
λ/4 (prior to coating)

Threading & Mounting

Mount Thickness (mm):
3.5 ±0.1

Environmental & Durability Factors

环境承受能力:
Humidity per MIL-STD-810H, Section 507.6
Temperature per MIL-STD-810H, Section 501.7 and 502.7

Additional Info

兼容的荧光团:
FITC Excitation

合规性

RoHS 2015:
Certificate of Conformance:
REACH 241:

产品系列说明

我们的 TECHSPEC® 荧光带通滤光片与荧光成像应用中的常见荧光团完美匹配。激发滤光片和发射滤光片具有>93%的透过率和>OD 6的带外截止。这些滤光片也是光谱学和临床化学应用以及生物技术仪器(如:DNA测序仪和聚合酶链反应(PCR)测试平台)的理想组件。每个滤光片都有加硬溅射镀膜,安装在一个黑色的阳极氧化环中。

关于每个激发或发射滤光片的兼容荧光团的完整列表,请查看技术文章《 荧光显微镜的荧光团和光学滤光片》。

Fluorescence microscopy and spectroscopy applications typically use a combination of three filters for each color channel (detection of individual fluorophores), an excitation and an emission bandpass filter, as well as a dichroic filter (also dichroic beamsplitters, such as our Fluorescence Dichroic Filters and High-Performance Fluorescence Dichroic Filters). More information on fluorescence microscopy and an example beam path can be found in the AppNote Fluorophores and Optical Filters for Fluorescence Microscopy. In short, excitation filters “clean up” the excitation light used to excite the fluorophores in the sample. On the other hand, emission filters allow only the emitted fluorescence to pass through to the detector while effectively blocking the excitation light and different wavelengths, producing high-contrast images and superior image clarity.

Our fluorescence bandpass filters are engineered with cutting-edge technology and are designed to enhance signal-to-noise ratios, improve contrast, and deliver excellent performance in optical imaging applications. These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for various imaging applications, including widefield, confocal, multiphoton, and other fluorescence microscopy modalities. Whether you are imaging fixed cells, live cells, or tissues, our filters are engineered to deliver consistent and reliable image quality. Additionally, these filters can be used for fluorescence detection in qPCR, flow cytometry, or multi-well plate readers in applications such as immunofluorescence or high-throughput screening in which fluorescent molecules or dyes detect specific molecular structures or DNA sequences.

TECHSPEC® Fluorescence Bandpass Filters are optimized for maximum transmission within a specific wavelength range. They effectively isolate fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.

All mounted TECHSPEC® Optical Filters have an arrow on the side of the mount that points to the filter-coated surface for quick reference. Filter oriented such that arrow points to filter coated surface S1. Anti-reflective (AR) coating is applied to S2.
All mounted TECHSPEC® Optical Filters have an arrow on the side of the mount that points to the filter-coated surface for quick reference. Filter oriented such that arrow points to filter coated surface S1. Anti-reflective (AR) coating is applied to S2.
Filter

Confocal Microscopy

Confocal microscopy provides high resolution, elimination of out-of-focus glare due to spatial filtering, and reduction of light-induced damage to the sample.

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Custom Bandpass Filter using Shortpass and Longpass Filters

Do stock filters not meet your bandwidth or CWL requirements? Learn how custom bandpass filters are created using short or longpass filters at Edmund Optics.

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Optical Filter Orientation

Unsure which side of your filter should face the light source? Learn how to determine the filter orientation of your TECHSPEC filter at Edmund Optics.

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滤光片介绍

Optical Filters are used to selectively transmit or block certain wavelengths or wavelength ranges within optical systems.

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滤光片镀膜-传统镀膜和硬质溅射镀膜的比较

Confused about the differences between traditional-coated and hard-coated optical filters?

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Selecting Color Filter Glass for Life Science Applications

What type of material should I look for in a filter?

How are notch filters designed and manufactured?

Bandpass Filter

Bandwidth

Cavity

Dichroic Coating

Dichroic Filter

Filter, Colored Glass

Ripple

光学滤光片的未来

New optical filters are being developed due to the advancements in applications and technologies. Learn more about the future of filters at Edmund Optics.

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荧光团和光学滤光片以进行荧光显微

Want to know more about fluorophores and optical filters for fluorescence microscopy? Find out more information and in stock optical filters at Edmund Optics.

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光学滤光片

Do you want to learn about optical filters? Find terminology, fabrication techniques, a selection guide, and application examples at Edmund Optics.

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Filtering in Machine Vision

While working with machine vision, there are different types of filters that can be used to alter the image. Find out about the different types at Edmund Optics.

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偏振片介绍

Polarizers are used in a wide range of imaging and research and development applications.

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Manipulating Wavebands: Color & Filters

Nick Sischka explains why color consideration and illumination are so important for machine vision in this hands-on demonstration from the Edmund Optics 2021 Imaging Innovation Summit.

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What happens when a filter is tilted?

How do I determine which side of my filter is coated?

Blocking

Blocking Range

Central Wavelength (CWL)

Cold Mirror

Full Width at Half Maximum (FWHM)

Interference Filter

Notch Filter

Peak Transmittance

Rugate Notch Filter

Shortpass Filter

带有激光照明的荧光成像

Fluorescence imaging systems are composed of three major components, an illumination source, a photo-activated fluorophore sample, and detector.

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How do I clean my filters?

What is the largest diameter filter available from Edmund Optics®?

What are some of the most common filter substrates?

How do I select the right filter for my application?

What types of filters can Edmund Optics® manufacture?

Do you have mounts for square or rectangular optics?

Cut-Off Wavelength

Cut-On Wavelength

Filter

Fluorescent Filter

Optical Density (OD)

Stopband

Optical Microscopy Application: Fluorescence

Wondering how fluorescence microscopy works? Find out about the technique, systems, and more at Edmund Optics.

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Basic Principles of Raman Scattering and Spectroscopy

Raman spectroscopy is a technique used to identify the chemical composition of samples based on how light scatters off of them. Learn more & view related optics

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荧光显微镜:内置了照明和滤光片用于成像

Want to know about fluorescence microscopy? Read this article by a Biomedical Product Line Engineer at Edmund Optics to learn more.

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去除保护性塑料镀膜

A protective plastic coating protects a variety of our flat optics, such as optical windows, optical mirrors, and beamsplitters, from scratches during shipping.

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Parallelism

Surface Flatness

How do I clean my optics?

Clear Aperture (CA)

EO 全球制造基地

Edmund Optics® (EO) manufactures millions of precision optical components and subassemblies every year in our 5 global manufacturing facilities.

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爱特蒙特光学的计量技术:测量是制造的关键环节

Learn about the metrology that Edmund Optics® uses to guarantee the quality of all optical components and assemblies.

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