我们的 TECHSPEC® 荧光带通滤光片与荧光成像应用中的常见荧光团完美匹配。激发滤光片和发射滤光片具有>93%的透过率和>OD 6的带外截止。这些滤光片也是光谱学和临床化学应用以及生物技术仪器(如:DNA测序仪和聚合酶链反应(PCR)测试平台)的理想组件。每个滤光片都有加硬溅射镀膜,安装在一个黑色的阳极氧化环中。
关于每个激发或发射滤光片的兼容荧光团的完整列表,请查看技术文章《 荧光显微镜的荧光团和光学滤光片》。
Fluorescence microscopy and spectroscopy applications typically use a combination of three filters for each color channel (detection of individual fluorophores), an excitation and an emission bandpass filter, as well as a dichroic filter (also dichroic beamsplitters, such as our Fluorescence Dichroic Filters and High-Performance Fluorescence Dichroic Filters). More information on fluorescence microscopy and an example beam path can be found in the AppNote Fluorophores and Optical Filters for Fluorescence Microscopy. In short, excitation filters “clean up” the excitation light used to excite the fluorophores in the sample. On the other hand, emission filters allow only the emitted fluorescence to pass through to the detector while effectively blocking the excitation light and different wavelengths, producing high-contrast images and superior image clarity.
Our fluorescence bandpass filters are engineered with cutting-edge technology and are designed to enhance signal-to-noise ratios, improve contrast, and deliver excellent performance in optical imaging applications. These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for various imaging applications, including widefield, confocal, multiphoton, and other fluorescence microscopy modalities. Whether you are imaging fixed cells, live cells, or tissues, our filters are engineered to deliver consistent and reliable image quality. Additionally, these filters can be used for fluorescence detection in qPCR, flow cytometry, or multi-well plate readers in applications such as immunofluorescence or high-throughput screening in which fluorescent molecules or dyes detect specific molecular structures or DNA sequences.
TECHSPEC® Fluorescence Bandpass Filters are optimized for maximum transmission within a specific wavelength range. They effectively isolate fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.
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